schleiferi in vivo in the Galleria mellonella wax moth larva, and found that expression of key S. schleiferi AIP was able to completely abolish agr induction of an S. aureus demonstrated that this molecule was likely responsible for the inhibitory activity, and further proof was provided when pure synthetic S. Subsequent cloning and heterologous expression of the S. aureus strain encoding a constitutively active AIP receptor we show that the activity is mediated via agr. The dog pathogen, Staphylococcus schleiferi, expressed the most potent inhibitory activity and was active against all four agr classes found in S. Here we show that culture supernatants of 37 out of 52 staphylococcal isolates representing 17 different species inhibit S.
aureus, and show that this interaction may eventually lead to the identification of new anti-virulence candidates to target S. In this study we aimed to better understand the interaction between staphylococci and S.
agr loci are found also in other staphylococcal species and for Staphylococcus epidermidis, the encoded AIP represses expression of agr regulated virulence genes in S. aureus virulence is controlled by the agr quorum sensing system responding to secreted auto-inducing peptides (AIPs) sensed by AgrC, a two component histidine kinase. While most are non-pathogenic colonizers, Staphylococcus aureus is an opportunistic pathogen capable of causing severe infections. Staphylococci are associated with both humans and animals.
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